The men suffered stab wounds when two groups clashed off Hobart's Salamanca Place in the Morrison St car park about midnight on Saturday, police said.
A 20-year-old man died from his injuries, while a 23-year-old man was still in hospital being treated for non-life-threatening injuries on Sunday morning, AP reported.
Police said it appeared to be an "isolated incident" that was possibly linked to a previous incident involving a person from each group.
"The two victims were known to each other - we don't know if they were known to the offender," Detective Inspector Mark Burke told reporters on Sunday.
"We're working through a lot of CCTV footage, speaking to witnesses and trying to establish exactly how many people are involved."
But Dept Supt Burke emphasised that it could have been a targeted attack.
"Someone being murdered in a public place is never a nice thing but we believe it is potentially a targeted thing.
"It's not someone walking up to someone in the street and stabbing them."
MA/PR
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