Researchers culture eye stem cells on nano scaffolds

TEHRAN, Sep. 11 (MNA) – Iranian researchers synthesized nanofibrous scaffold made of polymer and studied its performance for the culture of eye stem cells and tissue engineering.

Tissue engineering is used as a new scientific field to cure or recover various organs of the body and damaged tissues. Scaffolds produced in tissue engineering are required to have appropriate physical and chemical surface properties so a desirable connection is made between the cells and the scaffold after the cell culture process.

Electrospinning and casting methods are the most popular methods to produce scaffolds. The aim of this study was to compare the two production methods by taking into consideration parameters such as durability, adhesion and cell proliferation.

According to Iran Nanotechnology Initiative Council (INIC), the use of tissue engineering scaffolds produced through electrospinning method decreases the cost but increases efficiency in adhesion and cell culture.

The conditions of three-dimensional cell culture are closer to in-vivo genesis in comparison with two-dimensional culture. In fact, nanofibrous scaffolds create an environment similar to the extracellular matrix of the body for the cell by forming a network of nanometric and dense fibers with numerous pores. The environment affects morphology, adhesion, arrangement, migration, proliferation, distinguish and performance of the cells.

In this research, two polymeric scaffolds were produced through electrospinning and casting methods, and the performance of the scaffolds in morphology and cell culture was compared. Results showed that scaffolds produced through electrospinning method have a better performance in comparison with those produced through casting method.

Results of the research have been published in Journal of Biomedical Materials Research Part A, vol. 103, issue 9, 2015, pp. 2952-2958.


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