The Piper-34 light aircraft was flying from the southwest coastal city of Guayaquil towards Santa Rosa, reported Xinhua, citing the agency statement.
The pilot and a passenger died in the accident, while the other passenger was injured and taken to a health center for treatment.
This is the fourth aircraft accident in the South American country in the last two months.
On April 26, a military helicopter crashed in the northeast province of Pastaza, leaving eight dead, and on May 6, a Navy helicopter crashed in the western coastal province of Santa Elena, killing two pilots.
Three days later, a Cessna aircraft suffered an incident during takeoff on a runway in Morona Santiago, but there were no victims.
AMK/PR